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trap5b  (Immunodiagnostic Systems)

 
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    Structured Review

    Immunodiagnostic Systems trap5b
    Trap5b, supplied by Immunodiagnostic Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap5b/trap5b/pm41438305-62-5-9
    Average 86 stars, based on 1 article reviews
    trap5b - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Regulation of bone homeostasis by MERTK and TYRO3.
    Article Snippet: .. Mouse P1NP (Novus biologicals) and Trap5b (immunodiagnostic systems) levels were determined in peripheral blood using ELISA kits according to the manufacturer’s instructions. .. Human Igλ concentration was assessed in blood plasma from myeloma-bearing mice according to the manufacturerʼs instructions (Human Lambda ELISA KIT; Bethyl Laboratories Inc, Montgomery, TX, USA).

    Article Title: Tributyltin Protects Against Ovariectomy-Induced Trabecular Bone Loss in C57BL/6J Mice with an Attenuated Effect in High Fat Fed Mice
    Article Snippet: .. Serum was collected at euthanasia and analyzed by ELISA or EIA for PINP (Rat/Mouse PINP EIA (AC-33F1), Immunodiagnostic Systems, Tyne &Wear, UK), Trap5b (Mouse Trap Assay (SB-TR103), IDS), CTX (RatLaps EIA (AC-06F1), IDS), and osteocalcin (Mouse Osteocalcin ELISA (31-OSTMS-E01), ALPCO). ..

    Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts
    Article Snippet: RT-qPCR was performed using a SYBR Green Master Mix (Applied Biosystems) with primer pairs for fibrosis-related and osteoclast-related genes, as well as β-actin (Actb) as internal controls. .. The levels of analytes in mouse pleural lavage fluid and serum were measured by ELISA as follows: TRAP5b, Mouse TRAPTM (TRAP5b) ELISA Kit (Immunodiagnostic Systems, United Kingdom); IL-1β, Mouse IL-1β DuoSet ELISA Kit (R&D Systems Inc); CRP, Mouse C-Reactive Protein DuoSet ELISA Kit (R&D Systems Inc); RANKL, Mouse TRANCE/RANKL/TNFSF11 Quantikine ELISA Kit (R&D Systems Inc); OPG, Mouse Osteoprotegerin/TNFRSF11B DuoSet ELISA Kit (R&D Systems Inc); M-CSF, Mouse M-CSF DuoSet ELISA Kit (R&D Systems Inc) according to the manufacturer’s instructions. ..

    Article Title: Regulation of bone homeostasis by MERTK and TYRO3
    Article Snippet: .. Mouse P1NP (Novus biologicals) and Trap5b (immunodiagnostic systems) levels were determined in peripheral blood using ELISA kits according to the manufacturer’s instructions. .. Human Igλ concentration was assessed in blood plasma from myeloma-bearing mice according to the manufacturerʼs instructions (Human Lambda ELISA KIT; Bethyl Laboratories Inc, Montgomery, TX, USA).

    Article Title: Erythropoietin and bone health: Single high-dose administration triggers bone loss in mice.
    Article Snippet: Antibodies for Western blot detection of M-CSF and GAPDH were purchased from Abcam (Cambridge, UK). .. ELISA kits for P1NP and TRAP5b were purchased from Immunodiagnostic Systems (UK). .. An ELISA kit for M-CSF was purchased from ABclonal (USA).

    TRAP Assay:

    Article Title: Tributyltin Protects Against Ovariectomy-Induced Trabecular Bone Loss in C57BL/6J Mice with an Attenuated Effect in High Fat Fed Mice
    Article Snippet: .. Serum was collected at euthanasia and analyzed by ELISA or EIA for PINP (Rat/Mouse PINP EIA (AC-33F1), Immunodiagnostic Systems, Tyne &Wear, UK), Trap5b (Mouse Trap Assay (SB-TR103), IDS), CTX (RatLaps EIA (AC-06F1), IDS), and osteocalcin (Mouse Osteocalcin ELISA (31-OSTMS-E01), ALPCO). ..

    Marker:

    Article Title: iTr35 cells: a novel immunoregulatory subset involved in bone loss in rheumatoid arthritis and clinical implications
    Article Snippet: .. The markers included: TRAP5b (Tartrate-resistant acid phosphatase 5b): marker of osteoclast activity (SB-TR201A, Immunodiagnostic Systems, UK), CTX-I (C-terminal telopeptide of type I collagen): marker of bone resorption (CSB-E11224h, Cusabio Biotech, China); Osteocalcin: marker of bone formation (SEA471Ga, Cloud-Clone Corp, USA). ..

    Activity Assay:

    Article Title: iTr35 cells: a novel immunoregulatory subset involved in bone loss in rheumatoid arthritis and clinical implications
    Article Snippet: .. The markers included: TRAP5b (Tartrate-resistant acid phosphatase 5b): marker of osteoclast activity (SB-TR201A, Immunodiagnostic Systems, UK), CTX-I (C-terminal telopeptide of type I collagen): marker of bone resorption (CSB-E11224h, Cusabio Biotech, China); Osteocalcin: marker of bone formation (SEA471Ga, Cloud-Clone Corp, USA). ..



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    A Representative fluorescence images of the osteoclast phagocytosis assay in bone marrow–derived macrophages. BMM were stimulated with macrophage colony-stimulating factor (M-CSF), apoptotic thymocytes (APO), and receptor activator of nuclear factor kappa-B ligand (RANKL). Apoptotic thymocytes were labeled with CFDA (green), cytoplasm with CellMask Orange (orange), and nuclei with Hoechst 33342 (blue). Separate channels and merged overlays are shown. Cells with two nuclei were classified as pre-osteoclasts, and cells with three or more nuclei as osteoclasts. B Relative changes in the number, perimeter, and area of multinucleated TRAP+ cells after differentiation, with or without APO and transforming growth factor beta1 (TGF-β1) inhibitor. C Representative images of tartrate-resistant acid phosphatase (TRAP)-stained osteoclast cultures. D <t>TRAP5b</t> and E TGF-β protein expression in the supernatant of osteoclast cultures. F Analysis of relative gene expression of Nfatc1 (major transcription factor in osteoclastogenesis) , Acp5 (TRAP critical for osteoclastogenesis), and Rank (a receptor specific to osteoclasts and progenitors) gene. Statistical analysis involved a one-way ANOVA followed by Tukey’s multiple post hoc test. Data are presented as mean ± SD, and significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001 ( n = 3–8).
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    A Representative fluorescence images of the osteoclast phagocytosis assay in bone marrow–derived macrophages. BMM were stimulated with macrophage colony-stimulating factor (M-CSF), apoptotic thymocytes (APO), and receptor activator of nuclear factor kappa-B ligand (RANKL). Apoptotic thymocytes were labeled with CFDA (green), cytoplasm with CellMask Orange (orange), and nuclei with Hoechst 33342 (blue). Separate channels and merged overlays are shown. Cells with two nuclei were classified as pre-osteoclasts, and cells with three or more nuclei as osteoclasts. B Relative changes in the number, perimeter, and area of multinucleated TRAP+ cells after differentiation, with or without APO and transforming growth factor beta1 (TGF-β1) inhibitor. C Representative images of tartrate-resistant acid phosphatase (TRAP)-stained osteoclast cultures. D <t>TRAP5b</t> and E TGF-β protein expression in the supernatant of osteoclast cultures. F Analysis of relative gene expression of Nfatc1 (major transcription factor in osteoclastogenesis) , Acp5 (TRAP critical for osteoclastogenesis), and Rank (a receptor specific to osteoclasts and progenitors) gene. Statistical analysis involved a one-way ANOVA followed by Tukey’s multiple post hoc test. Data are presented as mean ± SD, and significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001 ( n = 3–8).
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    A Representative fluorescence images of the osteoclast phagocytosis assay in bone marrow–derived macrophages. BMM were stimulated with macrophage colony-stimulating factor (M-CSF), apoptotic thymocytes (APO), and receptor activator of nuclear factor kappa-B ligand (RANKL). Apoptotic thymocytes were labeled with CFDA (green), cytoplasm with CellMask Orange (orange), and nuclei with Hoechst 33342 (blue). Separate channels and merged overlays are shown. Cells with two nuclei were classified as pre-osteoclasts, and cells with three or more nuclei as osteoclasts. B Relative changes in the number, perimeter, and area of multinucleated TRAP+ cells after differentiation, with or without APO and transforming growth factor beta1 (TGF-β1) inhibitor. C Representative images of tartrate-resistant acid phosphatase (TRAP)-stained osteoclast cultures. D <t>TRAP5b</t> and E TGF-β protein expression in the supernatant of osteoclast cultures. F Analysis of relative gene expression of Nfatc1 (major transcription factor in osteoclastogenesis) , Acp5 (TRAP critical for osteoclastogenesis), and Rank (a receptor specific to osteoclasts and progenitors) gene. Statistical analysis involved a one-way ANOVA followed by Tukey’s multiple post hoc test. Data are presented as mean ± SD, and significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001 ( n = 3–8).
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    A Representative fluorescence images of the osteoclast phagocytosis assay in bone marrow–derived macrophages. BMM were stimulated with macrophage colony-stimulating factor (M-CSF), apoptotic thymocytes (APO), and receptor activator of nuclear factor kappa-B ligand (RANKL). Apoptotic thymocytes were labeled with CFDA (green), cytoplasm with CellMask Orange (orange), and nuclei with Hoechst 33342 (blue). Separate channels and merged overlays are shown. Cells with two nuclei were classified as pre-osteoclasts, and cells with three or more nuclei as osteoclasts. B Relative changes in the number, perimeter, and area of multinucleated TRAP+ cells after differentiation, with or without APO and transforming growth factor beta1 (TGF-β1) inhibitor. C Representative images of tartrate-resistant acid phosphatase (TRAP)-stained osteoclast cultures. D <t>TRAP5b</t> and E TGF-β protein expression in the supernatant of osteoclast cultures. F Analysis of relative gene expression of Nfatc1 (major transcription factor in osteoclastogenesis) , Acp5 (TRAP critical for osteoclastogenesis), and Rank (a receptor specific to osteoclasts and progenitors) gene. Statistical analysis involved a one-way ANOVA followed by Tukey’s multiple post hoc test. Data are presented as mean ± SD, and significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001 ( n = 3–8).
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    Image Search Results


    A Representative fluorescence images of the osteoclast phagocytosis assay in bone marrow–derived macrophages. BMM were stimulated with macrophage colony-stimulating factor (M-CSF), apoptotic thymocytes (APO), and receptor activator of nuclear factor kappa-B ligand (RANKL). Apoptotic thymocytes were labeled with CFDA (green), cytoplasm with CellMask Orange (orange), and nuclei with Hoechst 33342 (blue). Separate channels and merged overlays are shown. Cells with two nuclei were classified as pre-osteoclasts, and cells with three or more nuclei as osteoclasts. B Relative changes in the number, perimeter, and area of multinucleated TRAP+ cells after differentiation, with or without APO and transforming growth factor beta1 (TGF-β1) inhibitor. C Representative images of tartrate-resistant acid phosphatase (TRAP)-stained osteoclast cultures. D TRAP5b and E TGF-β protein expression in the supernatant of osteoclast cultures. F Analysis of relative gene expression of Nfatc1 (major transcription factor in osteoclastogenesis) , Acp5 (TRAP critical for osteoclastogenesis), and Rank (a receptor specific to osteoclasts and progenitors) gene. Statistical analysis involved a one-way ANOVA followed by Tukey’s multiple post hoc test. Data are presented as mean ± SD, and significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001 ( n = 3–8).

    Journal: Cell Death & Disease

    Article Title: Disrupted bone microenvironment and immune recovery following total body irradiation in a murine model

    doi: 10.1038/s41419-025-08303-7

    Figure Lengend Snippet: A Representative fluorescence images of the osteoclast phagocytosis assay in bone marrow–derived macrophages. BMM were stimulated with macrophage colony-stimulating factor (M-CSF), apoptotic thymocytes (APO), and receptor activator of nuclear factor kappa-B ligand (RANKL). Apoptotic thymocytes were labeled with CFDA (green), cytoplasm with CellMask Orange (orange), and nuclei with Hoechst 33342 (blue). Separate channels and merged overlays are shown. Cells with two nuclei were classified as pre-osteoclasts, and cells with three or more nuclei as osteoclasts. B Relative changes in the number, perimeter, and area of multinucleated TRAP+ cells after differentiation, with or without APO and transforming growth factor beta1 (TGF-β1) inhibitor. C Representative images of tartrate-resistant acid phosphatase (TRAP)-stained osteoclast cultures. D TRAP5b and E TGF-β protein expression in the supernatant of osteoclast cultures. F Analysis of relative gene expression of Nfatc1 (major transcription factor in osteoclastogenesis) , Acp5 (TRAP critical for osteoclastogenesis), and Rank (a receptor specific to osteoclasts and progenitors) gene. Statistical analysis involved a one-way ANOVA followed by Tukey’s multiple post hoc test. Data are presented as mean ± SD, and significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001 ( n = 3–8).

    Article Snippet: After 3 days of cultivation, TGF-β1 protein expression (Invitrogen 88-50690-22) and TRAP5b activity (Bone TRAP, Immunodiagnostic Systems) were measured in the culture supernatant from BMM differentiated osteoclasts.

    Techniques: Fluorescence, Phagocytosis Assay, Derivative Assay, Labeling, Staining, Expressing, Gene Expression